结构抗震能力评估计算程序的编制

简述了基于强度与延性理论的钢筋混凝土框架结构抗震能力评估方法,给出了抗震能力评估步骤,详细说明了为实现抗震能力的定量评估所编制的程序的主要功能及其编程思想。实例评估表明,编制的抗震能力评估计算程序,紧密结合我国建筑抗震设计规范,与ETABS集成软件结合,既可定量评估建筑结构抗震能力,也可从评估结果中了解造成抗震能力不足的原因,为抗震能力评估提供了新的思路。     

WGC特征描述的人脸表情识别

针对韦伯局部特征(WLD)仅计算中心像素与周围像素差异提取特征的不足,提出了一种韦伯梯度编码(WGC)特征描述的人脸表情识别算法。首先计算当前像素点周围水平、垂直和对角位置上的数值差与当前像素点的差异构成WGC特征的差动激励;然后进一步提出基于水平和对角线优先原则的WGC_HD特征;最后利用最佳分块方式得到行分块WGC_HD特征,采用自动优化参数的SVM分类器完成人脸表情识别。在公共人脸表情库JAFFE和CK库上进行交叉实验,平均识别率及平均特征提取时间分别为95.49%、12.30 ms和97.63%、31.54 ms。行分块WGC_HD特征考虑了不同梯度方向的像素差异,较好描述了表情图像的局部结构信息且具有较低的时间复杂度,与目前典型的表情识别算法结果对比也验证了算法具有较高的识别精度。     

基于正则表达式的变电站集中监控信息解析方法

随着国家电网公司"大运行"体系建设的深入开展,海量、繁杂且存在不规则现象的集中监控信息的识别,已成为设备监控信息智能告警技术提升面临的主要问题。文中结合集中监控信息既定规则的特点,提出一种基于正则表达式描述的集中监控信息解析方法。通过正则表达式规则提取信息关键要素进行信息解析,并采用最大分词匹配算法进行信息识别匹配,实现变电站集中监控信息实时解析及自动校验,为设备监控信息智能告警技术的进一步提升奠定了良好基础。实际工程应用验证了所提方法有效性和实用性。     

基于Volterra 级数对火工品起爆过程的辨识

为解决火工品起爆过程中线性函数不能解决元件参数变化、不确定性和非线性强的问题,将Volterra 级 数模型与基因表达式编程(gene expression programing,GEP)相结合,设计一种新的火工品起爆过程辨识算法。利用 Volterra 级数能准确反应非线性系统的特征来描述火工品起爆过程,GEP 算法则克服传统辨识方法的不足,辨识出正 确的模型。仿真结果表明,该方法能精确、快速地辨识出火工品起爆过程。     

适度设计——中国联合工程公司国机研发中心设计

结合中国联合工程公司国机研发中心设计,提出了"适度设计"的概念,探讨了建筑表达、高效空间、人文关怀以及低碳技术等内容。     

从三维模型到三维数字地图的突破——解读浙江省《三维数字地图技术规范》

三维数字产品已成为基础地理信息数据的重要组成部分,从三维模型到三维数字地图是个重大突破。本文简述了浙江省《三维数字地图技术规范》(DB33/T934-2014)的内容,分析了该标准与国家行业标准的关系以及在模型分类和编码、地图分幅和编号、比例尺、数学基础、精度和表达等方面的创新点。在实施时宜结合实际,选择产品的类型,制定相应的技术指标。     

An efficient small‐delay faults simulator based on critical path tracing

Testing for small‐delay defects (SDDs) has become an important component of integrated circuit testing. In this paper, an efficient small‐delay fault simulator, a hybrid method combining forward serial simulation and backward critical path tracing simulation for SDDs is proposed, which aims to determine the coverage of small‐delay defects for a given test set fast and accurately. In our proposed method, a unit delay model is employed, and reconvergent sensitization as well as hazard‐based detection is considered. Signal waveforms are expressed by bitmap data forms. In addition to providing an accurate result for fault simulation, the proposed simulator can well assist test generation. Experimental results demonstrate that the proposed simulator can further accelerate the simulation by one or two orders of magnitude compared with previous works. Copyright © 2014 John Wiley & Sons, Ltd.     

中蜂囊状幼虫病毒VP1基因的优化表达及其免疫原性分析

目的原核表达优化的中蜂囊状幼虫病毒(Chinese sacbrood virus,CSBV)的VP1基因,并分析其免疫原性。方法根据大肠埃希菌密码子偏爱性对野生型CSBV VP1(wt VP1)基因进行优化(opti VP1),将基因wt VP1和opti VP1分别克隆至p GEX-6P-1原核表达系统,构建原核表达质粒p GEX-6P-wt VP1和p GEX-6P-opti VP1,分别转化E.coli BL21,经IPTG诱导表达,并对诱导温度、IPTG终浓度及诱导时间进行优化,诱导蛋白经Western blot鉴定;重组蛋白经GSH-琼脂糖凝胶层析纯化;分别用PBS、纯化的GST标签蛋白、纯化的重组蛋白及CSBV纯化病毒免疫小鼠,经间接ELISA法检验各组小鼠的血清抗体效价。结果优化后的VP1基因序列降低了密码子影响指数,提高了密码子适应指数;质粒p GEX-6P-wt VP1和p GEX-6P-opti VP1经鉴定证明构建正确;最佳诱导表达条件为:IPTG终浓度0.8 mmol/L,30℃,220 r/min诱导8 h;诱导表达蛋白占菌体总蛋白百分比由wt VP1菌株(5.49±0.30)%提高至opti VP1菌株(59.7±0.51)%,纯化后纯度可达0.5 mg/ml;兔抗GST标签抗体及兔抗CSBV血清均与优化后的重组蛋白发生特异性反应;重组蛋白组小鼠二免后可产生较高水平抗体,明显高于PBS组和GST标签蛋白组(P0.05)。结论通过密码子优化实现了CSBV结构基因VP1的高效表达,表达蛋白可诱导小鼠机体产生特异性抗体,具有良好的免疫原性,为探讨CSBV感染的分子发病机制和制备抗CSBV病毒的多克隆抗体奠定了基础。     

Individual trans 18:1 Isomers are Metabolised Differently and Have Distinct Effects on Lipogenesis in 3T3‐L1 Adipocytes

The objective of this research was to study the metabolism of individual trans fatty acids (FAs) that can be found in ruminant fat or partially hydrogenated vegetable oils (PHVO) and determine their effects on FA composition and lipogenic gene expression in adipocytes. Differentiated 3T3‐L1 adipocytes were treated with 200 µM of either trans‐9‐18:1, trans‐11‐18:1, trans‐13‐18:1, cis‐9‐18:1 or BSA vehicle control for 120 h. Trans‐9‐18:1 increased total cell FA content (µmole/well) compared to other FA treatments, which was mainly related to the accumulation of trans‐9‐18:1 in the cells. Adipocytes were able to desaturate a significant proportion of absorbed trans‐11‐18:1 and trans‐13‐18:1 (~20 and 30 % respectively) to cis‐9,trans‐11‐18:2 and cis‐9,trans‐13‐18:2, whereas trans‐9‐18:1 was mostly incorporated intact resulting in a greater lipophilic index (i.e. decreased mean FA fluidity) of adipocytes. Trans‐9‐18:1 up‐regulated (P < 0.05) the expression of lipogenic genes including acetyl‐CoA carboxylase (1.65 fold), FA synthase (1.45 fold), FA elongase‐5 (1.52 fold) and stearoyl‐CoA desaturase‐1 (1.49 fold), compared to the control, whereas trans‐11‐18:1 and trans‐13‐18:1 did not affect the expression of these genes compared to control. Our results suggest that the metabolism and lipogenic properties of trans‐11‐18:1 and trans‐13‐18:1, typically the most abundant trans FA in beef from cattle fed forage‐based diets, are similar and are different from those of trans‐9‐18:1, the predominant trans FA in PHVO.     

Molecular Cloning and Functional Characterization of Mannose Receptor in Zebra Fish (Danio rerio) during Infection with Aeromonas sobria

Mannose receptor (MR) is a member of pattern-recognition receptors (PRRs), which plays a significant role in immunity responses. Much work on MR has been done in mammals and birds while little in fish. In this report, a MR gene (designated as zfMR) was cloned from zebra fish (Danio rerio), which is an attractive model for the studies of animal diseases. The full-length cDNA of zfMR contains 6248 bp encoding a putative protein of 1428 amino acids. The predicted amino acid sequences showed that zfMR contained a cysteine-rich domain, a single fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short C-terminal cytoplasmic domain, sharing highly conserved structures with MRs from the other species. The MR mRNA could be detected in all examined tissues with highest level in kidney. The temporal expression patterns of MR, IL-1β and TNF-α mRNAs were analyzed in the liver, spleen, kidney and intestine post of infection with Aeromonas sobria. By immunohistochemistry assay, slight enhancement of MR protein was also observed in the spleen and intestine of the infected zebra fish. The established zebra fish-A. sobria infection model will be valuable for elucidating the role of MR in fish immune responses to infection.